The Structure and Function of Horseradish Peroxidase (HRP) under the Influence of Faradarmani Consciousness Field

Abstract

The study of biological enzymes with their unique properties, such as substrate interactions, conformational changes, and catalytic rates, is a key to basic and applied enzymology research. Among enzymes, horseradish peroxidase (HRP) has been widely used in studying the metabolism of biological systems. In this study, we measure changes in the activity and structure of horseradish peroxidase in both laboratory grade and isoforms extracted from the plant under the influence of Faradarmani Consciousness Field (FCF). Our objectives were first to test the effect of FCF on the structure and function of HRP and, secondly, to analyze whether or not changes in the maximum velocity and Michaelis constant were reproducible under the influence of FCF. We observed that the laboratory-grade HRP had a 5% decrease in maximum velocity and an 18% increase in Michaelis constant as a result of the FCF treatment when compared to the non-treated controls. On the other hand, the HRP extracted from the plant tissue showed a 6% and 40% increase in maximum velocity and Michaelis constant, respectively, under FCF. Additionally, changes in the fluorescence emission intensity spectrum suggest that both structural and functional changes in this enzyme result from the FCF treatment.