Effects of Faradarmani Consciousness Field on Hippocampal Structure/Function and Pancreatic Beta Cells in STZ-Induced Diabetic Rats

Abstract

Diabetes mellitus (DM) is a growing health issue that harms brain structure and function, particularly in the hippocampus, which is crucial for learning and memory and is affected by disrupted glucose homeostasis. There are limited non-invasive treatments specifically targeting CNS impairments associated with diabetes. T-Consciousness Fields (TCFs) with non-physical entities have been introduced by Taheri. The influence of these fields can be investigated on various subjects, including living organisms and inanimate materials. The purpose of this study is to investigate the effect of a type of TCFs named the Faradarmani Consciousness Field on hippocampus structure and function in diabetic rats. The male Wistar rats (n= 48, 150±10 g weight, 8 weeks age) were categorized into four groups (n=12) including control (C), Faradarmani Consciousness Field (T), Diabetic control (D), Faradaemani Consciousness Field +diabetic (DT). Faradarmani was applied over eight weeks to both Diabetic (DT) and T groups. Peritoneal injection of STZ solution (50 mg/kg) was used to induce diabetes. Cognitive functions were assessed using the Morris water maze. At the end of the experiment, rats were sacrificed, and their brains were processed for cresyl violet staining to quantify surviving neurons in the hippocampus. H&E staining showed significant disorganization and cell loss in brain regions of the diabetic group. According to cresyl staining, in diabetic groups, Faradarmani treatment significantly reduced the death of cells in the CA3 and DG areas of hippocampus. Additionally, the rate of beta cell destruction and cellular apoptosis, as graded, decreased in the DT group but not significantly. Data from the Morris water maze revealed that diabetic groups exhibited notably impaired cognitive performance relative to the non-diabetic group. The T group spent significantly more time in the target zone across all days, while the DT group outperformed the D group on the second day. Although the distance traveled was significantly higher in the Faradarmani groups, the time spent in target zone for the T and DT groups suggests a possible improvement in memory retention. In conclusion, this experiment provides evidence of the effects of Faradarmani on structure and function of hippocampus in a rat model.