Cell Cycle Progression of Jurkat (Leukemia) and LA- N-5 (Neuroblastoma) Cell Lines under the Influence of Taheri Consciousness Fields

Abstract

Mohammad Ali Taheri has introduced T-consciousness as a third element of the universe, in addition to matter and energy. There is a wide variety of Taheri Consciousness Fields (TCFs) that cannot be measured directly. However, it is possible to investigate their effects on various subjects. Previously, survival and death of different kinds of cancer cell lines have been evaluated under influence of TCFs. This experiment was designed with the aim of reproducing obtained results and investigating the effects of two types of TCFs (1 and 2) on this behavior of Jurkat cell line with lymphoblast morphology and LA-N-5 cell line with fibroblast morphology, which cause leukemia and neuroblastoma, respectively. First, the effects of TCFs on the Jurkat cell line was examined using the MTT assay at 12, 24, and 48 hours. Additionally, after 48 hours, cell cycle analysis was performed in both cell lines using flow cytometry. According to the obtained data, TCF2 increased the viability of the Jurkat cell line at 12 hours, while TCF1 caused a significant increase in the G2/M phase, which may suggest checkpoint activation and accumulation of cells in this phase under the influence of the field. For LA-N-5, the results showed that TCF2 led to a significant increase in the S phase percentage. This increase was accompanied by a decrease in the G2/M phase, which may indicate an S-phase arrest. However, TCF1 did not induce any significant changes. These observations indicate that the application of different TCFs can lead to varying outcomes. Further studies are required to clarify the effects of TCFs at the biological levels.