Effects of Taheri Consciousness Fields on the HT29 Human Colorectal Cancer Cells

Colorectal cancer accounts for 11% of all diagnosed cancers and is the second deadliest cancer worldwide. Surgery, chemotherapy and targeted therapy are generally used for the treatment of colorectal cancer. However, there are significant challenges, such as recurrence of the tumor and drug resistance, so the application of novel methods is required for the treatment of this cancer. T-Consciousness Fields (TCFs) were introduced by Mohammad Ali Taheri. These new fields are neither energy nor matter and cannot be measured directly. However, we can evaluate the effects of TCFs indirectly through various kinds of research in the laboratory. This study aimed to evaluate the effect of TCF-1 and TCF-2 on HT29 human colon cancer cells compared with the control group. The morphology and microscopic properties of cells were investigated in the TCF-1 and TCF-2 groups compared with the control, and the cells were detached and stained with trypan blue, and then the dead cells were counted. To evaluate the effect of the TCFs on cell viability, the MTT assay was used. The expression of two apoptosis-related genes (Bax and Bcl-2) was assessed using reverse transcription quantitative PCR (RT-qPCR). The results showed that TCFs decreased the number of cells and changed the morphology of HT29 cells. In the trypan blue staining method, more dead cells were counted in the TCF groups compared to the control group. In the MTT assay, both TCFs decreased the viability of cells during incubation times (p < 0.05). Bax/Bcl2 ratio increased 4.9-fold and 7.6-fold in TCF-1 and TCF-2 treated cells, respectively (p < 0.05). Therefore, TCF-1 and TCF-2 induced apoptosis in HT29 cells. There are still many ambiguities and questions about the nature and effect of TCFs. To clarify this issue, more research is needed in vitro, in vivo, and clinically.