Effects of Taheri Consciousness Fields on the Rat C6 Glioma Cell Line

The most common primary spinal cord and brain tumors are gliomas, which represent 81% of malignant brain tumors. In neuro-oncology investigations, the rat C6 glioma cell line has been used most often as an experimental model. Taheri Consciousness Fields (TCFs), introduced by Mohammad Ali Taheri, are novel fields that are neither matter nor energy. Therefore, they are non-quantifiable and cannot be directly observed or measured. However, it is possible to demonstrate and measure the effects of these fields through standard scientific experiments. This study aimed to evaluate the effects of TCF-1 and TCF-2 on the C6 glioma cell line, compared with the untreated control group. The morphology and microscopic characteristics of the cells were evaluated. Also, trypsinized and detached cells were stained with trypan blue, and then dead cells were counted. To evaluate the effect of TCFs on cell viability, the MTT assay was used. The expression of Bax and Bcl-2 genes was assessed using reverse transcription quantitative PCR (RT-qPCR). The results showed that TCFs treatment not only reduced the number of C6 glioma cells but also changed their morphology. Similarly, in the trypan blue staining method, more dead cells were counted in the TCF groups compared to the control group. In the MTT assay, both TCFs exhibited cytotoxic effects on C6 glioma cells at the incubation times tested (p < 0.05). Bax/Bcl2 ratio increased 3.5-fold and 7-fold compared to the control via TCF-1 and TCF-2, respectively (p < 0.05). The findings suggest that TCF-1 and TCF-2 can induce apoptosis in rat C6 glioma cells. The mechanism of action of TCFs is still not well understood, and studies of the effects of TCFs in vivo and in clinical research could be useful to clarify their therapeutic effects.