Effect of the Faradarmani Consciousness Field on immune response induced by an inactivated vaccine against Foot and Mouth disease virus (FMDV) in rats and replication of FMDV in vitro

Foot-and-mouth disease (FMD) is one of the highest-risk factors affecting the animal industry worldwide. Currently, available commercial FMD vaccines have numerous limitations, such as slow induction and short-term maintenance of antibody titers. Therefore, a novel approach is needed that can induce high neutralizing antibody titers to protect the host in the early stages of FMD virus (FMDV) infection and maintain high antibody titers for extended periods after a single vaccination dose. There are several T-Consciousness Fields (TCFs), introduced by Mohammad Ali Taheri. TCFs are not matter or energy, so they cannot be measured directly. However, we can indirectly evaluate their effects through several reproducible laboratory experiments. The present study aimed to evaluate the effect of Faradarmani CF, a type of the TCFs, on FMDV replication, titer, and RNA copy number as well as the humoral immune response against two types of inactivated FMDV vaccines with different adjuvants in rats. Two types of FMD vaccines with different adjuvants (Freund's and Alum) were prepared, and 30 male Wistar rats were immunized with the vaccines. Rats were divided into six groups (n=5 per group). Four groups of rats were studied using different treatment combinations, and two groups served as positive and negative controls. Vaccination intervals were every 14 days, three times. Serum neutralization test (SN) and Enzyme-linked immunosorbent assay (ELISA) were used to assess changes in antibody levels in rat serum samples after each immunization. The results showed that Faradarmani CF induced viral replication in vitro. In addition, antibody levels in treated groups under both Freund's adjuvant and Alum adjuvant vaccines increased significantly compared to groups without Faradarmani CF treatment. In conclusion, our data suggest that Faradarmani CF may offer a practical approach to improving immunization and vaccination success against FMDV serotype O. It is recommended that the effects of TCFs across different vaccine types be investigated.